Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
Summary: Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory.This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling).Here, we describe a protocol to monitor hydrangea red sensation actin filament retrograde flow and actin poly